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Home > Products > Product Lineup > MagDEA® DNA 200 (GC)

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MagDEA® DNA 200 (GC)

Product Description

MagDEA® DNA 200 (GC) is a genomic DNA extraction reagents for automated nucleic acid extraction system Magtration® Systems 6GC, 12GC and 12GC PLUS. The dedicated pre-filled reagents cartridge contains all reagents used in extraction and purification of DNA from samples and enables without special skill to operate extraction easily used with recommended 2-propanol and dedicated consumables. The high purity of DNA extracted can be used directly in the followed step such as PCR, restriction enzyme reaction or sequencing reaction. In addition, all consumables (tips, tip holders and screw cap tubes) needed in extraction are included in the reagents kit so that extraction can be done after purchase.

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Feartures

  • Direct extraction and purification of genomic DNA from whole blood samples.
  • The volume of elution is selectable 100μl or 50μl.
  • High purified DNA allowing use of the product in PCR, restriction enzyme reaction and sequencing reaction is possible.
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Specification

Sample volume 200μl
Sample type

Whole blood(*), Cell, E.Coli, etc.

*Use IC card of "MagDEA® DNA 200 Whole Blood" if sample is whole blood.

Yield of gDNA 200μl of whole blood (containing EDTA-2Na): approx. 5μg
*The value depends on the sample
A260/A280 1.9~2.0
Processing time

Whole Blood : Approximately 40min

Non-whole blood sample : Approximately 50min

Instrument Magtration® System 6GC/12GC/12GC PLUS
Storage condition Room temperature(15~25°C)
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Reference Data

The various genomic DNA were extracted from each human whole blood by using different anticoagulants and also by using MagDEA® DNA 200 (GC) by Magtration® System 12GC. PCR reaction was performed by using the extracted genomic DNA as template in a thermal cycler "DICE" (manufactured by Takara Bio Inc.) and the presence of the amplification products was examined by agarose gel electrophoresis. As a result, favorable amplification products were obtained from all extracted genome DNAs. (Fig.1)

Fig.1

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Ordering Information

Product NameProduct CodePackageQuick manualData sheet
MagDEA® DNA 200 (GC) E7001 48 tests/kit *

(Whole Blood Protocol)

(Non-whole blood Protocol)

PDF

*The kit contains tips (50), tip holders (50) and screw cap tubes (200).
*2-Propanol is not included in the reagent kit. (Click here to see recommended product.)

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Protocol IC Card

Product NameProduct CodePackage
MagDEA DNA 200 Whole Blood 6GC I7601 1
MagDEA DNA 200 6GC I7691 1
MagDEA DNA 200 Whole Blood 12GC I7201 1
MagDEA DNA 200 12GC I7291 1
MagDEA DNA 200 Whole Blood PLUS I7501 1
MagDEA DNA 200 PLUS I7591 1
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Related Consumables

*The kit contains tips (50), tip holders (50) and screw cap tubes (200).

Product NameProduct CodePackageRemark
GC series Tip set F4300 300 set
(300 × Tip, 300 × Tip sheath)
Set of Tips and sheath for Magtration® System 6G, 12GC and 12GC Plus.
GC series processing cartridge F4301 350 pieces Reaction cartridge for Magtration® System 6GC, 12GC and 12GC Plus
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Recommended Consumables

• 2-Propanol
Wako Chemicals, 2-Propanol (JIS Special Grade)
Cat. No.: 166-04831 or 166-04836

SIGMA-Aldrich, 2-Propanol ACS reagent, >99.5%
Cat. No.: 190764

Fluka, 2-Propanol LC-MS CHROMASOLV, ≥99.9%
Cat. No.: 34965

MERCK, 2-Propanol
Cat. No.: 818766 or 101040

Serva Electrophoresis GmbH, Isopropanol molecular biology grade
Cat. No.: 39559.02

Alfa Aesar (Lancaster), 2-Propanol 99+%
Cat. No.: L10181

Alfa Aesar (ALF), 2-Propanol 99.5+%
Cat. No.: 041840

Note; Use 2-Propanol sealed in glass bottle.

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Correspondance instrument

Product NameProduct CodePackageRemark
Magtration® System 6GC A1002 1 unit Automated nucleic acid extraction system allowing simultaneous processing of up to 6 samples.
Magtration® System 12GC PLUS A1500 1 unit Automated nucleic acid extraction system allowing simultaneous processing of up to 12 samples.
Optionally, the system may contain a bar code control system.

*This product will be discontinued in October 31, 2016. Please see our official notification.

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Click here to see FAQ

*Magtration® is a PSS patented technology which is applied in more than ten countries including Japan, the United States and Europe.

(Japanese Patent: No.3115501; US Patent: No. 5,702,950; European Patent No.687501)

*Magtration® is a registered trademark of Precision System Science, Co., Ltd.

*Information and specifications of the products in this page are subject to change without prior notice for improvement.

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