MagDEA® DNA Whole Blood (8Lx) is a genomic DNA extraction reagent from 7ml or 5ml whole blood sample, dedicated for automated nucleic acid extraction system Magtration® System 8Lx. The dedicated pre-filled reagents cartridge contains all reagents needed for DNA extraction and purification in whole blood and enables without special skill to operate extraction easily used with recommended 2-propanol and dedicated consumables. The high purity of DNA extracted can be used directly in the followed step such as PCR, restriction enzyme reaction or sequencing reaction. Since consumables needed for extraction (syringe tips, BT holders, 2.0 ml screw cap tubes, 30 ml round-bottomed test tubes, etc.) and 2-propanol are not included in the kit. They should be prepared separately.
*This product was discontinued in March 2016.
|Sample volume||7ml or 5ml|
|Sample type||Human whole blood|
|Yield of gDNA||
7ml of human whole blood (containing EDTA-2Na): approx. 120-400 μg
5ml of human whole blood (containing EDTA-2Na): approx. 80-290 μg
|Processing time||Approximately 70min|
|Instrument||Magtration® System 8Lx|
|Storage condition||Room temperature (15~25°C)|
Genomic DNAs were extracted from 7 ml human whole blood by using different anticoagulants and by using MagDEA® DNA Whole Blood (8Lx) and Magtration® System 8Lx. PCR reaction was performed by using the extracted genomic DNA as template in a thermal cycler "DICE" (manufactured by Takara Bio Inc.) and the presence of the amplification products was examined by agarose gel electrophoresis. As a result, favorable amplification products were obtained from all extracted genomic DNAs.(Fig.1)
|Product Name||Product Code||Package||Quick manual||Data sheet|
|MagDEA® DNA Whole Blood (8Lx)||E7002||8tests/kit*|
*Various consumables (syringe tips, tubes, etc.) are not included in the reagent kit.
*2-Propanol is not included in the reagent kit. Click here to see recommended consumables.
|Product Name||Product Code||Package||Remark|
|F8000||48 pcs||Processing tip for Magtration® System 8Lx.|
|BT holder||F8002||16 pcs||Sample tube (blood collection tube) holder for Magtration® System 8Lx|
|Bar-code Tag||F8001||100 pcs||Barcode tag for elution tube.
*If you don't manage the elution tube ID, no tag is needed.
Product of Wako Pure Chemical Industries, Ltd.
2-Propanol (JIS Special Grade)
Cat. No.: 166-04831 or 166-04836
Product of SIGMA-Aldrich
2-Propanol ACS reagent, >99.5%
Cat. No.: 190764
Note; Use 2-Propanol sealed in glass bottle.
• Sample tube (Blood collection tube)
[For 7ml sample volume]
VENOJECT II for 7ml
Product code; VP-**070K or VP-**070KN
[For 5ml sample volume]
VENOJECT II for 5ml
Product code; VP-**050K or VP-**050KN
• Elution tube (Eluate collection tube)
2.0ml Screw cap micro tube
Product code; 72.693
• Reaction tube (for Heat block)
Product code; 55.517
|Magtration® System 8Lx||A1008||1 unit||Large volume automated nucleic acid extraction system allowing simultaneous processing up to 8 samples.|
*Magtration® is a PSS patented technology which is applied in more than ten countries including Japan, the United States and Europe.
(Japanese Patent: No.3115501; US Patent: No. 5,702,950; European Patent No.687501)
*Magtration® is a registered trademark of Precision System Science, Co., Ltd.
*Information and specifications of the products in this page are subject to change without prior notice for improvement.