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Home > Products > Product Lineup > MagDEA® DNA Whole Blood (8Lx)

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MagDEA® DNA Whole Blood (8Lx)

Product Description

MagDEA® DNA Whole Blood (8Lx) is a genomic DNA extraction reagent from 7ml or 5ml whole blood sample, dedicated for automated nucleic acid extraction system Magtration® System 8Lx. The dedicated pre-filled reagents cartridge contains all reagents needed for DNA extraction and purification in whole blood and enables without special skill to operate extraction easily used with recommended 2-propanol and dedicated consumables. The high purity of DNA extracted can be used directly in the followed step such as PCR, restriction enzyme reaction or sequencing reaction. Since consumables needed for extraction (syringe tips, BT holders, 2.0 ml screw cap tubes, 30 ml round-bottomed test tubes, etc.) and 2-propanol are not included in the kit. They should be prepared separately.

*This product was discontinued in March 2016.

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Feartures

  • Direct extraction and purification of genomic DNA (without pretreatment) through blood collecting tube.
  • The elution volume is 1 ml.
  • High purified DNA allowing use of the product in PCR, restriction enzyme reaction and sequencing reaction is possible.
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Specification

Sample volume 7ml or 5ml
Sample type Human whole blood
Yield of gDNA

7ml of human whole blood (containing EDTA-2Na): approx. 120-400 μg

5ml of human whole blood (containing EDTA-2Na): approx. 80-290 μg
*The value depends on the sample

A260/A280 1.8~2.0
Processing time Approximately 70min
Instrument Magtration® System 8Lx
Storage condition Room temperature (15~25°C)
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Reference Data

Genomic DNAs were extracted from 7 ml human whole blood by using different anticoagulants and by using MagDEA® DNA Whole Blood (8Lx) and Magtration® System 8Lx. PCR reaction was performed by using the extracted genomic DNA as template in a thermal cycler "DICE" (manufactured by Takara Bio Inc.) and the presence of the amplification products was examined by agarose gel electrophoresis. As a result, favorable amplification products were obtained from all extracted genomic DNAs.(Fig.1)

Fig.1

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Ordering Information

Product NameProduct CodePackageQuick manualData sheet
MagDEA® DNA Whole Blood (8Lx) E7002 8tests/kit* PDF

*Various consumables (syringe tips, tubes, etc.) are not included in the reagent kit.
*2-Propanol is not included in the reagent kit. Click here to see recommended consumables.

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Related Consumables

Product NameProduct CodePackageRemark
Syringe Tip
(non-sterile)
F8000 48 pcs Processing tip for Magtration® System 8Lx.
BT holder F8002 16 pcs Sample tube (blood collection tube) holder for Magtration® System 8Lx
Bar-code Tag F8001 100 pcs Barcode tag for elution tube.
*If you don't manage the elution tube ID, no tag is needed.
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Recommended Consumables

• 2-Propanol
Product of Wako Pure Chemical Industries, Ltd.
2-Propanol (JIS Special Grade)
Cat. No.: 166-04831 or 166-04836

Product of SIGMA-Aldrich
2-Propanol ACS reagent, >99.5%
Cat. No.: 190764

Note; Use 2-Propanol sealed in glass bottle.

• Sample tube (Blood collection tube)
[For 7ml sample volume]
TERUMO Corporation
VENOJECT II for 7ml
Product code; VP-**070K or VP-**070KN

[For 5ml sample volume]
TERUMO Corporation
VENOJECT II for 5ml
Product code; VP-**050K or VP-**050KN

• Elution tube (Eluate collection tube)
SARSTEDT
2.0ml Screw cap micro tube
Product code; 72.693

• Reaction tube (for Heat block)
SARSTEDT
30ml tube
Product code; 55.517

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Correspondance instrument

Product NameCodePackageRemark
Magtration® System 8Lx A1008 1 unit Large volume automated nucleic acid extraction system allowing simultaneous processing up to 8 samples.
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Click here to see FAQ

*Magtration® is a PSS patented technology which is applied in more than ten countries including Japan, the United States and Europe.

(Japanese Patent: No.3115501; US Patent: No. 5,702,950; European Patent No.687501)

*Magtration® is a registered trademark of Precision System Science, Co., Ltd.

*Information and specifications of the products in this page are subject to change without prior notice for improvement.

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