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Home > Products > Product Lineup > MagDEA® RNA 100 (GC)

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MagDEA® RNA 100 (GC)
E7004

Product Description

MagDEA® RNA 100 (GC) is a dedicated reagent for extracting total RNA from sample for automated nucleic acid extraction system Magtration® Systems 6GC, 12GC and 12GC PLUS. The pre-filled reagents cartridge contains all needed for extraction and purification of Total RNA from homogenates of samples such as cells and tissues are previously placed in a single reagent cartridge, as they are separated. The reagents cartridge enables without special skill to operate extraction easily used with recommended 2-propanol and dedicated consumables. The high purity total RNA extracted can be used directly in the followed step such as PCR, RT-PCR and real-time PCR reaction. In addition, all consumables (tips, tip holders and screw cap tubes) needed in extraction are included in the reagents kit so that extraction can be done after purchase.

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Feartures

  • Extraction and purification of total RNA from cells and tissues.
  • The elution volume is selectable to 100μl or 50μl.
  • DNase treatment can be selected (DNase is not included).
  • High purified nucleic acid allowing use of the product in PCR, RT-PCR and real-time PCR.
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Specification

Sample volume 100ul
Sample type Cell, Tissue
Yield

10mg Mouse liver: approximately 25ug

*The value depends on sample condition.

A260/A280 1.9~2.0
Processing time

approximately 40min (without DNase treatment)

approximately 65min (with DNase treatment)

Correspondence instrument Magtration® System 6GC/12GC/12GC PLUS
Storage condition Room temperature(15~25°C)
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Reference Data

Total RNAs were extracted from mouse liver and mouse kidney by using MagDEA® RNA 100 (GC) and Magtration® System 12GC. One-step RT-PCR reaction was carried out by using the extracted total RNA as template and DICE (manufactured by Takara Bio Inc.) and the presence of the amplification products was examined by agarose gel electrophoresis. As a result, there were observed stabilized amplification products from all extracted total RNAs. (Fig.1)

Fig.1

E7004_fig
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Ordering Information

Product NameProduct CodePackageQuick manualData sheet
MagDEA® RNA 100 (GC) E7004 48tests/kit* PDF

*The kit contains tips (50), tip holders (50) and screw cap tubes (200).
*2-Propanol is not included in the reagent kit. (Click here to see recommended product.)

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Protocol IC Card

Product NameProduct CodePackage
MagDEA RNA 100 6GC I7604 1
MagDEA RNA 100 12GC I7204 1
MagDEA RNA 100 PLUS I7504 1
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Related Consumables

*The kit contains tips (50), tip holders (50) and screw cap tubes (200).

Product NameProduct CodePackageRemark
GC series Tip set F4300 300 set
(300 × Tip, 300 × Tip sheath)
Set of Tips and sheath for Magtration® System 6G, 12GC and 12GC PLUS.
GC series processing cartridge F4301 350 pieces Reaction cartridge for Magtration® System 6GC, 12GC and 12GC PLUS.
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Recommended Consumables

• 2-Propanol
Wako Chemicals, 2-Propanol (JIS Special Grade)
Cat. No.: 166-04831 or 166-04836

SIGMA-Aldrich, 2-Propanol ACS reagent, >99.5%
Cat. No.: 190764

Fluka, 2-Propanol LC-MS CHROMASOLV, ≥99.9%
Cat. No.: 34965

MERCK, 2-Propanol
Cat. No.: 818766 or 101040

Serva Electrophoresis GmbH, Isopropanol molecular biology grade
Cat. No.: 39559.02

Alfa Aesar (Lancaster), 2-Propanol 99+%
Cat. No.: L10181

Alfa Aesar (ALF), 2-Propanol 99.5+%
Cat. No.: 041840

Note; Use 2-Propanol sealed in glass bottle.

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Correspondance instrument

Product NameProduct CodePackageRemark
Magtration® System 6GC A1002 1 unit Automated nucleic acid extraction system allowing simultaneous processing of up to 6 samples.
Magtration® System 12GC PLUS A1500 1 unit Automated nucleic acid extraction system allowing simultaneous processing of up to 12 samples.
Optionally, the system may contain a bar code control system.

*This product will be discontinued in October 31, 2016. Please see our official notification.

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Click here to see FAQ

*Magtration® is a PSS patented technology which is applied in more than ten countries including Japan, the United States and Europe.

(Japanese Patent: No.3115501; US Patent: No. 5,702,950; European Patent No.687501)

*Magtration® is a registered trademark of Precision System Science, Co., Ltd.

*Information and specifications of the products in this page are subject to change without prior notice for improvement.

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