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Home > Products > Product Lineup > MagDEA Dx SV

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MagDEA® Dx SV
E7004

Product Description

MagDEA Dx SV is a nucleic acid extraction reagent developed with fully automated systems in mind. All extraction reagents come sealed in cartridges with everything including magnetic particles needed for extraction of nucleic acid (DNA/RNA). By combining with PSS Magtration Platforms, preparation of high quality nucleic acids is simple, efficient and accurate.
The instrument and reagent were also each designed in accordance with the European IVD Directive (98/79/EC) for safe operation, high quality, and user convenience.

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Feartures

  • Universal design, able to handle various sample types and sample volumes
  • Can be used with multiple instruments according to your purpose
  • Designed in accordance with the European IVD Directive (98/79/EC)
  • Ready to be used pre-filled reagent
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Specification

Extraction sample volumeElution volumeInstrumentsExtraction timeTest / kit
magDEA Dx SV 200-400 μL 50 / 100 / 200 μL magLEAD 6gC
magLEAD 12gC
geneLEAD XII plus
approximately 25 mins 48 test / kit
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Reference Data

•Linearity test of extracts from various sample matrices, spiked with M13 DNA bacteriophage

Table 1. The linearity test analysis showing obtained Ct values from seven different human sample matrices

Serum Plasma Plasma Swab Swab CSF Urine
(EDTA) (ACD) (Throat) (nasal)
Slope -3.447 -3.406 -3.415 -3.369 -3.391 -3.361 -3.397
correlation coefficient (R2) 0.995 0.997 0.998 0.999 0.999 0.996 0.998
PCR efficiency (%) 95.027 96.594 96.253 98.061 97.215 98.405 96.954
y-intercept 41.863 41.556 41.782 41.097 41.463 40.883 41.052

Figure 1

Figure 1. Linearity plot graph showing copy numbers and Ct values from M13 bacteriophage included serum as typical data.

M13 DNA bacteriophage, using 10 μL and 7 different concentrations, were added to 200 μL of following human body fluid samples; Serum, plasma (EDTA-2Na), plasma (ACD), swab (throat), swab (nasal), cerebrospinal fluid (CSF) and urine. Those samples were prepared to final numbers of 1×10 2, 3, 4, 5, 6, 7 and 8 copies/extraction (5 replicates). DNA was extracted using MagDEA® Dx SV on geneLEAD XII plus, and the extracts were PCR amplified using ABI 7500 Dx fast (M13 specific TaqMan probe). 100 copies/extraction were detected in all tests. Slopes, correlation coefficient (R2), PCR efficiency and y-intercept were calculated using the Ct values obtained from samples between 1x102-8 copies/extraction (Table 1). No differences were observed between the sample species. PCR amplification curve and Linearity plot graph using serum is shown in Figure 1.

•Genomic DNA from human whole blood

Figure 2

Figure 2. The yield, A260/280, and A260/230 of genomic DNA from whole blood sample.

Genomic DNA was extracted from human EDTA-2Na (specimen A) or ACD (specimen B) whole blood samples using MagDEA® Dx SV for total 6 days (6 replicates by each run). The white blood cell (WBC) numbers of specimen A and B were 6.4 and 9.2 k/μL respectively. The concentrations and purities of the extracts were measured using ND-1000 spectrometer (NanoDrop) (Figure 2). After 6 runs, there were no significant variations between the two specimens.

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Ordering Information

Product NameProduct CodePackageQuick manualMSDS

MagDEA Dx SV

E1300

48 test Contact us

PDF

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Related Consumables

for magLEAD 6gC / magLEAD 12gC

Product NameProduct CodePackageRemark

magLEAD Consumable Kit

F4430

50 tests/kit

Disposable set necessary for extraction

for geneLEAD XII plus

Product NameProduct CodePackageRemark

geneLEAD Consumable Set

F2118

48 tests/kit

geneLEAD PCR Cassette

F2121

192 tests/kit

geneLEAD Waste Box

F2125

20 pc/kit

for magLEAD 5bL

Product NameProduct CodePackageRemark

magLEAD Tip LV

Contact us

30 tests/kit

Bellows Tip

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Correspondance instrument

MagDEA Dx SV

Product NameProduct CodePackage
magLEAD 6gC A1060 1set
magLEAD 12gC A1220 1set
geneLEAD XII plus A2603 1set

MagDEA Dx LV

Product NameProduct CodePackage
magLEAD 5bL Contact us 1set
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For more information and about purchase, please contact us by telephone or inquiry form.

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*Magtration® and MagDEA Dx® is a PSS patented technology which is applied in more than ten countries including Japan, the United States and Europe.
(Japanese Patent: No.3115501; US Patent: No.5,702,950; European Patent: No.687501)

*Magtration® is a registered trademark of Precision System Science, Co., Ltd.

*Information and specifications of the products in this page are subject to change without prior notice for improvement.

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