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Home > Technologies > PSS Technologies > Column Tip Technology

Technologies

Column Tip Technology

Column Tip technology

Applications of Column Tip

PSS has developed Various Column Tips for maximumizing performance of the carrier sealed in the column tip. Combination of the Column Tip with the Magtration® tip would enable automation of purification and concentration of samples in various fields.

  • Magtration® tip for handling of liquid and magnetic particles
  • Affinity column tip containing a carrier for adsorption of proteins and nucleic acids
  • Gel filtration column tip for gel filtration of separating proteins and nucleic acids by size
  • Filter tip for separation and concentration by using a membrane filter or an ultra-filtration membrane
Genetic field
(1)+(3): Magtration® tip for handling of liquid and magnetic particles
(1)+(4): Nucleic acid extraction or PCR clean up --> Concentration or high-purity purification (complete removal of few magnetic particles)
Application (1)+(3):  Sample preparation flow chart for gene analysis using nucleic acid extraction with magnetic particles using the Magtration® tip and the PCR clean up with filter tip
Proteome field
(1)+(3) Extraction/purification of fusion proteins --> buffer exchange (demineralization)
(1)+(4) Extraction/purification of fusion proteins --> molecular sieve (removal of protein polymer)
(1)+(3)+(1) Removal of abundant proteins --> buffer exchange (demineralization) --> enzyme digestion (trypsin-immobilized magnetic beads)
(2)+(3) Removal of abundant proteins --> buffer exchange (demineralization), affinity purification --> buffer exchange
(2)+(3)+(4) Removal of abundant proteins --> buffer exchange (demineralization) --> concentration, affinity purification --> buffer exchange --> concentration, target capture --> buffer exchange (demineralization) --> concentration
(2)+(3)+(4)+(1) Removal of abundant proteins --> buffer exchange (demineralization) --> concentration --> enzyme digestion (trypsin-immobilized magnetic beads)
Application (2)+(3)+(4):  Sample preparation flow chart for protein analysis using affinity column tip, chromatography column tip and filter tip
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