Multiplex Assay Technology "BIST®"

BIST® for protein analysis
BIST® used in DNA analysis

Multiplex Assay Technology by APiT®

BIST® Technology for Multiplex signal detection

BIST® (Beads array In Single Tip) is a multiplex detection tool developed for automated simultaneous multi-item measurements.

1mm diameter beads coated with a single immobilized antibody, antigen or DNA fragment are lined up inside the BIST® tip. Sample is automatically pipetted through the tip, binding target substances to the beads. After an enzymatic reaction, signals can be detected and measured simultaneously from up to 20 different bead-targets.

PSS will support your application development using BIST®, including reagent prepacking, reaction process and detection automation.

BIST® Technology video

Examples of multiplex assays suitable for BIST® analysis

Assay Items Sample
Body testing (Obesity factors) β3AR、UCP1、AGT235、MTHFR  
Application to health guidance, talent analysis, etc.    
Allergic reaction tests Cedar, cypress, ragweed, HD (tick, cat, dog etc.)  
Cancer markers AFP (hepatoma), PSA (prostatic cancer), p53 antibody  
Cancer-related tests HLA Typing, sugar chain detection  
Cardiac disease marker Myoglobin, troponin, PCT  
Clinical testing items TSH, Free T3, Free T4, AFP, albumin  
Infant mass screening TSH, 17-OHP, Free T4 Dried blood spot
Autoimmune diseases    
Pharmacogenomics(PGx) IK28, SYP2C19, EGFR (Iressa target agent)  
Immunodeficiency disease CD3 (T lymphocytes), CD19 (B lymphocytes), CD45 (leukocyte)  
Food allergens Wheat, buckwheat, peanuts, eggs, milk, shrimp, crab Food
Genetically modified food (GMO) protein Herbicide resistant enzyme PAT, CP4-EPSPS Food (corn, soy beans)
Genetically modified food (GMO) genes Specific gene detection Food (corn, soy beans)
Crop production location identification, variety identification Tuna variety identification, cashmere identification Meat, hair, etc.
Food poisoning Salmonella (bacteria) Stool, food
Staphylococcus aureus (bacteria, toxins: enterotoxins)
Vibrio parahaemolyticus (bacteria)
Bacillus cereus (bacteria, toxins)
E. coli (bacteria, toxins)
Legal communicable disease Cholera, dysentery, typhoid Stool, food
Diarrheal viruses Norovirus, rotavirus, adenovirus
Enterohaemorrhagic E. coli O157, O26
Infectious disease Mumps, EV virus  
Water examination Legionella Water
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